Journal: BMC Cell Biology

Article Title: Hydrophobicity of protein determinants influences the recognition of substrates by EDEM1 and EDEM2 in human cells

doi: 10.1186/s12860-015-0047-7

Figure Lengend Snippet: BACE457 DHF is a membrane protein. (A) Cells were subjected to permeabilisation (see ) with increasing concentrations of digitonin, concentrations as indicated. A representative membranes of cytosolic fraction and membrane fraction after Western blot with anti-calnexin (clx) or anti-calreticulin (cln) antibodies are shown. (B) Cells were transfected with cDNA encoding BACE457, BACE457 DHF or BACE457Δ and subjected to permeabilisation with increasing concentrations of digitonin, concentrations as indicated. A representative membranes of cytosolic fraction and membrane fraction after Western blot with anti-BACE antibodies are shown. (C) Cells were transfected with cDNA encoding BACE457, BACE457 DHF or BACE457Δ and subjected to permeabilisation with 60 μg/ml of digitonin. Then cells were fixed and stained as indicated in Methods. Bars, 10 μm.

Article Snippet: The mouse anti-calnexin were obtained from BD Biosciences (Palo Alto, CA), anti-calreticulin from BioSite (San Diego, CA).

Techniques: Western Blot, Transfection, Staining

Journal: PLoS ONE

Article Title: Identification of CREB3L1 as a Biomarker Predicting Doxorubicin Treatment Outcome

doi: 10.1371/journal.pone.0129233

Figure Lengend Snippet: (A and B) Whole cell lysate (A) or indicated fractions (B) of the RCC xenograft tumors harvested 3 days after the initial injection (2 mice for each tumor) were subjected to immunoblot analysis with the indicated antibodies. Calnexin, lysine-specific demethylase 1 (LSD1) and actin served as loading controls. (C-L) Tumors harvested 21 days after the initial injection were subjected to IHC with anti-endomucin. Representative blood vessels marked by endomucin staining are indicated by arrows. Scale bar: 100 μm. (M) Quantification of blood vessel density in each line of the tumors determined by IHC of endomucin. For each individual tumor, five independent images were taken for calculation. For each line of tumors, results are reported as mean ± S.E. from n ≥ 4 tumors taken from different mice.

Article Snippet: We obtained mouse anti-γH2AX from Millipore; peroxidase-conjugated secondary antibodies from Jackson ImmunoResearch; rabbit anti-Actin and Doxorubicin (Cat# D1515-10MG) from Sigma-Aldrich; Rapamycin from LC Laboratories; rabbit anti-LSD1 from Cell Signaling; mouse anti-calnexin from Enzo Life Sciences.

Techniques: Injection, Western Blot, Staining

Journal: Frontiers in Cellular Neuroscience

Article Title: Arc Interacts with the Integral Endoplasmic Reticulum Protein, Calnexin

doi: 10.3389/fncel.2017.00294

Figure Lengend Snippet: Antibodies used in this study (IF, immunofluorescence; IP, immunoprecipitation; WB, western blot).

Article Snippet: Mouse anti-calnexin C-term , IF1:200 , Abnova M08 , AB_1137121.

Techniques: Immunofluorescence, Immunoprecipitation, Western Blot